An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera: Symphyta)

Robert I. Graham, Viviane Zahner, Christopher J. Lucarotti

Research output: Contribution to journalJournal Article

Abstract

Six species of sawfly (Hymenoptera: Symphyta) from four taxonomic families (Agridae, Diprionidae, Pamphiliidae, and Tenthredinidae) were collected from locations across Canada and surveyed for their associated microbiota. Total DNA was extracted from individual insects, and polymerase chain reaction (PCR) was used to amplify the conserved 16S rRNA gene from microbiota. Denaturing gradient gel electrophoresis (DGGE) and restriction fragment length polymorphism (RFLP) were undertaken to separate bacterial clones associated with the host insect. Sequencing of the PCR-DGGE and PCR--RFLP products revealed a dominance of alpha- and gamma-Proteobacteria, with most sequences showing high similarity to bacteria previously identified from other insect species and environmental samples. Additionally, a strain of the bacterial endosymbiont Wolbachia and a Wolbachia bacteriophage were identified from the mountain ash sawfly (Pristiphora geniculata).
Original languageEnglish
Pages (from-to)758-768
Number of pages11
JournalCanadian Journal of Microbiology
Volume54
Issue number9
DOIs
Publication statusPublished - Sep 2008
Externally publishedYes

Fingerprint

Hymenoptera
Microbiota
Wolbachia
Insects
Denaturing Gradient Gel Electrophoresis
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Sorbus
Population
Alphaproteobacteria
Gammaproteobacteria
rRNA Genes
Bacteriophages
Canada
Clone Cells
Bacteria
DNA

Keywords

  • Bacteria
  • Endosymbiont
  • Hymenoptera
  • Sawflies
  • Wolbachia

Cite this

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title = "An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera: Symphyta)",
abstract = "Six species of sawfly (Hymenoptera: Symphyta) from four taxonomic families (Agridae, Diprionidae, Pamphiliidae, and Tenthredinidae) were collected from locations across Canada and surveyed for their associated microbiota. Total DNA was extracted from individual insects, and polymerase chain reaction (PCR) was used to amplify the conserved 16S rRNA gene from microbiota. Denaturing gradient gel electrophoresis (DGGE) and restriction fragment length polymorphism (RFLP) were undertaken to separate bacterial clones associated with the host insect. Sequencing of the PCR-DGGE and PCR--RFLP products revealed a dominance of alpha- and gamma-Proteobacteria, with most sequences showing high similarity to bacteria previously identified from other insect species and environmental samples. Additionally, a strain of the bacterial endosymbiont Wolbachia and a Wolbachia bacteriophage were identified from the mountain ash sawfly (Pristiphora geniculata).",
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An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera: Symphyta). / Graham, Robert I.; Zahner, Viviane; Lucarotti, Christopher J.

In: Canadian Journal of Microbiology, Vol. 54, No. 9, 09.2008, p. 758-768.

Research output: Contribution to journalJournal Article

TY - JOUR

T1 - An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera: Symphyta)

AU - Graham, Robert I.

AU - Zahner, Viviane

AU - Lucarotti, Christopher J.

PY - 2008/9

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AB - Six species of sawfly (Hymenoptera: Symphyta) from four taxonomic families (Agridae, Diprionidae, Pamphiliidae, and Tenthredinidae) were collected from locations across Canada and surveyed for their associated microbiota. Total DNA was extracted from individual insects, and polymerase chain reaction (PCR) was used to amplify the conserved 16S rRNA gene from microbiota. Denaturing gradient gel electrophoresis (DGGE) and restriction fragment length polymorphism (RFLP) were undertaken to separate bacterial clones associated with the host insect. Sequencing of the PCR-DGGE and PCR--RFLP products revealed a dominance of alpha- and gamma-Proteobacteria, with most sequences showing high similarity to bacteria previously identified from other insect species and environmental samples. Additionally, a strain of the bacterial endosymbiont Wolbachia and a Wolbachia bacteriophage were identified from the mountain ash sawfly (Pristiphora geniculata).

KW - Bacteria

KW - Endosymbiont

KW - Hymenoptera

KW - Sawflies

KW - Wolbachia

U2 - 10.1139/W08-067

DO - 10.1139/W08-067

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JF - Canadian Journal of Microbiology

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