Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 758-768 |
| Number of pages | 11 |
| Journal | Canadian Journal of Microbiology |
| Volume | 54 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 2008 |
| Externally published | Yes |
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Keywords
- Bacteria
- Endosymbiont
- Hymenoptera
- Sawflies
- Wolbachia
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An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera: Symphyta). / Graham, Robert I.; Zahner, Viviane; Lucarotti, Christopher J.
In: Canadian Journal of Microbiology, Vol. 54, No. 9, 09.2008, p. 758-768.Research output: Contribution to journal › Journal Article
TY - JOUR
T1 - An intracellular symbiont and other microbiota associated with field-collected populations of sawflies (Hymenoptera: Symphyta)
AU - Graham, Robert I.
AU - Zahner, Viviane
AU - Lucarotti, Christopher J.
PY - 2008/9
Y1 - 2008/9
N2 - Six species of sawfly (Hymenoptera: Symphyta) from four taxonomic families (Agridae, Diprionidae, Pamphiliidae, and Tenthredinidae) were collected from locations across Canada and surveyed for their associated microbiota. Total DNA was extracted from individual insects, and polymerase chain reaction (PCR) was used to amplify the conserved 16S rRNA gene from microbiota. Denaturing gradient gel electrophoresis (DGGE) and restriction fragment length polymorphism (RFLP) were undertaken to separate bacterial clones associated with the host insect. Sequencing of the PCR-DGGE and PCR--RFLP products revealed a dominance of alpha- and gamma-Proteobacteria, with most sequences showing high similarity to bacteria previously identified from other insect species and environmental samples. Additionally, a strain of the bacterial endosymbiont Wolbachia and a Wolbachia bacteriophage were identified from the mountain ash sawfly (Pristiphora geniculata).
AB - Six species of sawfly (Hymenoptera: Symphyta) from four taxonomic families (Agridae, Diprionidae, Pamphiliidae, and Tenthredinidae) were collected from locations across Canada and surveyed for their associated microbiota. Total DNA was extracted from individual insects, and polymerase chain reaction (PCR) was used to amplify the conserved 16S rRNA gene from microbiota. Denaturing gradient gel electrophoresis (DGGE) and restriction fragment length polymorphism (RFLP) were undertaken to separate bacterial clones associated with the host insect. Sequencing of the PCR-DGGE and PCR--RFLP products revealed a dominance of alpha- and gamma-Proteobacteria, with most sequences showing high similarity to bacteria previously identified from other insect species and environmental samples. Additionally, a strain of the bacterial endosymbiont Wolbachia and a Wolbachia bacteriophage were identified from the mountain ash sawfly (Pristiphora geniculata).
KW - Bacteria
KW - Endosymbiont
KW - Hymenoptera
KW - Sawflies
KW - Wolbachia
U2 - 10.1139/W08-067
DO - 10.1139/W08-067
M3 - Journal Article
VL - 54
SP - 758
EP - 768
JO - Canadian Journal of Microbiology
JF - Canadian Journal of Microbiology
SN - 0008-4166
IS - 9
ER -