Characterization of a novel member of genus Iflavirus in Helicoverpa armigera

He Yuan, Pengjun Xu, Xianming Yang, Robert I. Graham, Kenneth Wilson, Kongming Wu

Research output: Contribution to journalJournal Article

6 Citations (Scopus)

Abstract

The cotton bollworm, Helicoverpa armigera, is one of the most important agricultural pests of many economic crops worldwide. Herein, we found a novel single-strand RNA virus by RNA-Seq and Polymerase Chain Reaction (PCR) method in H. armigera named Helicoverpa armigera iflavirus (HaIV), which possessed a genome with 10,017 nucleotides in length and contained a single large open reading frame (ORF) encoding a putative polyprotein of 3021 amino acids with a predicted molecular mass of 344.16 kDa and a theoretical isoelectric point (pI) of 6.45. The deduced amino acid sequence showed highest similarity (61.0%) with the protein of Lymantria dispar Iflavirus 1. Phylogenetic analysis with putative RdRp amino acid sequences indicated that the virus clustered with members of the genus Iflavirus. The virus was mainly distributed in the fat body of its host and was found to be capable of both horizontal and vertical transmission. The efficiency of perorally horizontal transmission was dose dependent (100% infection rate with a viral dose of 108 copies/μl) while vertical transmission efficiency was found to be relatively low (
Original languageEnglish
Pages (from-to)65-73
Number of pages9
JournalJournal of Invertebrate Pathology
Volume144
DOIs
Publication statusPublished - 1 Mar 2017
Externally publishedYes

Fingerprint

Iflavirus
Helicoverpa armigera
horizontal transmission
vertical transmission
virus
amino acid
RNA
amino acid sequences
viruses
polymerase chain reaction
fat
cotton
Lymantria dispar
fat body
isoelectric point
genome
dosage
phylogenetics
open reading frames
crop

Keywords

  • Helicoverpa armigera
  • Horizontal transmission
  • Iflavirus
  • RNA virus
  • Tissue distribution
  • Vertical transmission

Cite this

Yuan, He ; Xu, Pengjun ; Yang, Xianming ; Graham, Robert I. ; Wilson, Kenneth ; Wu, Kongming. / Characterization of a novel member of genus Iflavirus in Helicoverpa armigera. In: Journal of Invertebrate Pathology. 2017 ; Vol. 144. pp. 65-73.
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abstract = "The cotton bollworm, Helicoverpa armigera, is one of the most important agricultural pests of many economic crops worldwide. Herein, we found a novel single-strand RNA virus by RNA-Seq and Polymerase Chain Reaction (PCR) method in H. armigera named Helicoverpa armigera iflavirus (HaIV), which possessed a genome with 10,017 nucleotides in length and contained a single large open reading frame (ORF) encoding a putative polyprotein of 3021 amino acids with a predicted molecular mass of 344.16 kDa and a theoretical isoelectric point (pI) of 6.45. The deduced amino acid sequence showed highest similarity (61.0{\%}) with the protein of Lymantria dispar Iflavirus 1. Phylogenetic analysis with putative RdRp amino acid sequences indicated that the virus clustered with members of the genus Iflavirus. The virus was mainly distributed in the fat body of its host and was found to be capable of both horizontal and vertical transmission. The efficiency of perorally horizontal transmission was dose dependent (100{\%} infection rate with a viral dose of 108 copies/μl) while vertical transmission efficiency was found to be relatively low (",
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Characterization of a novel member of genus Iflavirus in Helicoverpa armigera. / Yuan, He; Xu, Pengjun; Yang, Xianming; Graham, Robert I.; Wilson, Kenneth; Wu, Kongming.

In: Journal of Invertebrate Pathology, Vol. 144, 01.03.2017, p. 65-73.

Research output: Contribution to journalJournal Article

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AU - Xu, Pengjun

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AU - Graham, Robert I.

AU - Wilson, Kenneth

AU - Wu, Kongming

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AB - The cotton bollworm, Helicoverpa armigera, is one of the most important agricultural pests of many economic crops worldwide. Herein, we found a novel single-strand RNA virus by RNA-Seq and Polymerase Chain Reaction (PCR) method in H. armigera named Helicoverpa armigera iflavirus (HaIV), which possessed a genome with 10,017 nucleotides in length and contained a single large open reading frame (ORF) encoding a putative polyprotein of 3021 amino acids with a predicted molecular mass of 344.16 kDa and a theoretical isoelectric point (pI) of 6.45. The deduced amino acid sequence showed highest similarity (61.0%) with the protein of Lymantria dispar Iflavirus 1. Phylogenetic analysis with putative RdRp amino acid sequences indicated that the virus clustered with members of the genus Iflavirus. The virus was mainly distributed in the fat body of its host and was found to be capable of both horizontal and vertical transmission. The efficiency of perorally horizontal transmission was dose dependent (100% infection rate with a viral dose of 108 copies/μl) while vertical transmission efficiency was found to be relatively low (

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