Complete sequence, analysis and organization of the orgyia leucostigma nucleopolyhedrovirus genome

David K. Thumbi, Robert J.M. Eveleigh, Christopher J. Lucarotti, Renée Lapointe, Robert I. Graham, Lillian Pavlik, Hilary A.M. Lauzon, Basil M. Arif

Research output: Contribution to journalJournal Article

11 Citations (Scopus)

Abstract

The complete genome of the Orgyia leucostigma nucleopolyhedrovirus (OrleNPV) isolated from the whitemarked tussock moth (Orgyia leucostigma, Lymantridae: Lepidoptera) was sequenced, analyzed, and compared to other baculovirus genomes. The size of the OrleNPV genome was 156,179 base pairs (bp) and had a G+C content of 39%. The genome encoded 135 putative open reading frames (ORFs), which occupied 79% of the entire genome sequence. Three inhibitor of apoptosis (ORFs 16, 43 and 63), and five baculovirus repeated ORFs (bro-a through bro-e) were interspersed in the OrleNPV genome. In addition to six direct repeat (drs), a common feature shared among most baculoviruses, OrleNPV genome contained three homologous regions (hrs) that are located in the latter half of the genome. The presence of an F-protein homologue and the results from phylogenetic analyses placed OrleNPV in the genus Alphabaculovirus, group II. Overall, OrleNPV appears to be most closely related to group II alphabaculoviruses Ectropis obliqua (EcobNPV), Apocheima cinerarium (ApciNPV), Euproctis pseudoconspersa (EupsNPV), and Clanis bilineata (ClbiNPV).
Original languageEnglish
Pages (from-to)2301-2327
Number of pages27
JournalViruses
Volume3
Issue number11
DOIs
Publication statusPublished - Nov 2011
Externally publishedYes

Fingerprint

Nucleopolyhedrovirus
Sequence Analysis
Genome
Baculoviridae
Open Reading Frames
Lepidoptera
Moths
Nucleic Acid Repetitive Sequences
Base Composition
Base Pairing
Apoptosis

Keywords

  • Alphabaculovirus
  • Baculovirus
  • White-marked tussock moth

Cite this

Thumbi, D. K., Eveleigh, R. J. M., Lucarotti, C. J., Lapointe, R., Graham, R. I., Pavlik, L., ... Arif, B. M. (2011). Complete sequence, analysis and organization of the orgyia leucostigma nucleopolyhedrovirus genome. Viruses, 3(11), 2301-2327. https://doi.org/10.3390/v3112301
Thumbi, David K. ; Eveleigh, Robert J.M. ; Lucarotti, Christopher J. ; Lapointe, Renée ; Graham, Robert I. ; Pavlik, Lillian ; Lauzon, Hilary A.M. ; Arif, Basil M. / Complete sequence, analysis and organization of the orgyia leucostigma nucleopolyhedrovirus genome. In: Viruses. 2011 ; Vol. 3, No. 11. pp. 2301-2327.
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abstract = "The complete genome of the Orgyia leucostigma nucleopolyhedrovirus (OrleNPV) isolated from the whitemarked tussock moth (Orgyia leucostigma, Lymantridae: Lepidoptera) was sequenced, analyzed, and compared to other baculovirus genomes. The size of the OrleNPV genome was 156,179 base pairs (bp) and had a G+C content of 39{\%}. The genome encoded 135 putative open reading frames (ORFs), which occupied 79{\%} of the entire genome sequence. Three inhibitor of apoptosis (ORFs 16, 43 and 63), and five baculovirus repeated ORFs (bro-a through bro-e) were interspersed in the OrleNPV genome. In addition to six direct repeat (drs), a common feature shared among most baculoviruses, OrleNPV genome contained three homologous regions (hrs) that are located in the latter half of the genome. The presence of an F-protein homologue and the results from phylogenetic analyses placed OrleNPV in the genus Alphabaculovirus, group II. Overall, OrleNPV appears to be most closely related to group II alphabaculoviruses Ectropis obliqua (EcobNPV), Apocheima cinerarium (ApciNPV), Euproctis pseudoconspersa (EupsNPV), and Clanis bilineata (ClbiNPV).",
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Thumbi, DK, Eveleigh, RJM, Lucarotti, CJ, Lapointe, R, Graham, RI, Pavlik, L, Lauzon, HAM & Arif, BM 2011, 'Complete sequence, analysis and organization of the orgyia leucostigma nucleopolyhedrovirus genome', Viruses, vol. 3, no. 11, pp. 2301-2327. https://doi.org/10.3390/v3112301

Complete sequence, analysis and organization of the orgyia leucostigma nucleopolyhedrovirus genome. / Thumbi, David K.; Eveleigh, Robert J.M.; Lucarotti, Christopher J.; Lapointe, Renée; Graham, Robert I.; Pavlik, Lillian; Lauzon, Hilary A.M.; Arif, Basil M.

In: Viruses, Vol. 3, No. 11, 11.2011, p. 2301-2327.

Research output: Contribution to journalJournal Article

TY - JOUR

T1 - Complete sequence, analysis and organization of the orgyia leucostigma nucleopolyhedrovirus genome

AU - Thumbi, David K.

AU - Eveleigh, Robert J.M.

AU - Lucarotti, Christopher J.

AU - Lapointe, Renée

AU - Graham, Robert I.

AU - Pavlik, Lillian

AU - Lauzon, Hilary A.M.

AU - Arif, Basil M.

PY - 2011/11

Y1 - 2011/11

N2 - The complete genome of the Orgyia leucostigma nucleopolyhedrovirus (OrleNPV) isolated from the whitemarked tussock moth (Orgyia leucostigma, Lymantridae: Lepidoptera) was sequenced, analyzed, and compared to other baculovirus genomes. The size of the OrleNPV genome was 156,179 base pairs (bp) and had a G+C content of 39%. The genome encoded 135 putative open reading frames (ORFs), which occupied 79% of the entire genome sequence. Three inhibitor of apoptosis (ORFs 16, 43 and 63), and five baculovirus repeated ORFs (bro-a through bro-e) were interspersed in the OrleNPV genome. In addition to six direct repeat (drs), a common feature shared among most baculoviruses, OrleNPV genome contained three homologous regions (hrs) that are located in the latter half of the genome. The presence of an F-protein homologue and the results from phylogenetic analyses placed OrleNPV in the genus Alphabaculovirus, group II. Overall, OrleNPV appears to be most closely related to group II alphabaculoviruses Ectropis obliqua (EcobNPV), Apocheima cinerarium (ApciNPV), Euproctis pseudoconspersa (EupsNPV), and Clanis bilineata (ClbiNPV).

AB - The complete genome of the Orgyia leucostigma nucleopolyhedrovirus (OrleNPV) isolated from the whitemarked tussock moth (Orgyia leucostigma, Lymantridae: Lepidoptera) was sequenced, analyzed, and compared to other baculovirus genomes. The size of the OrleNPV genome was 156,179 base pairs (bp) and had a G+C content of 39%. The genome encoded 135 putative open reading frames (ORFs), which occupied 79% of the entire genome sequence. Three inhibitor of apoptosis (ORFs 16, 43 and 63), and five baculovirus repeated ORFs (bro-a through bro-e) were interspersed in the OrleNPV genome. In addition to six direct repeat (drs), a common feature shared among most baculoviruses, OrleNPV genome contained three homologous regions (hrs) that are located in the latter half of the genome. The presence of an F-protein homologue and the results from phylogenetic analyses placed OrleNPV in the genus Alphabaculovirus, group II. Overall, OrleNPV appears to be most closely related to group II alphabaculoviruses Ectropis obliqua (EcobNPV), Apocheima cinerarium (ApciNPV), Euproctis pseudoconspersa (EupsNPV), and Clanis bilineata (ClbiNPV).

KW - Alphabaculovirus

KW - Baculovirus

KW - White-marked tussock moth

U2 - 10.3390/v3112301

DO - 10.3390/v3112301

M3 - Journal Article

VL - 3

SP - 2301

EP - 2327

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 11

ER -