Discovery and characterization of a novel picorna-like RNA virus in the cotton bollworm Helicoverpa armigera

Xianming Yang, Pengjun Xu, He Yuan, Robert I. Graham, Kenneth Wilson, Kongming Wu

Research output: Contribution to journalJournal Article

1 Citation (Scopus)

Abstract

We characterize a novel picorna-like virus, named Helicoverpa armigera Nora virus (HaNV), with a genome length of 11,200 nts, the sequence of which was isolated from the lepidopteran host cotton bollworm Helicoverpa armigera, using RNA-Seq. Phylogenetic analysis, using the putative amino acid sequence of the conserved RNA-dependent RNA polymerase (RdRp) domain, indicated that HaNV clustered with Spodoptera exigua Nora virus, Drosophila Nora virus and Nasonia vitripennis virus-3 with a high bootstrap value (100%), which might indicate a new viral family within the order Picornavirales. HaNV was efficiently horizontally transmitted between hosts via contaminated food, and transmission was found to be dose-dependent (up to 100% efficiency with 109 viral copy number/µl). HaNV was also found to be transmitted vertically from parent to offspring, mainly through transovum transmission (virus contamination on the surface of the eggs), but having a lower transmission efficiency (around 43%). Infection distribution within the host was also investigated, with HaNV mainly found in only the gut of both adult moths and larvae (>90%). Moreover, our results showed that HaNV appears not to be an overtly pathogenic virus to its host.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Invertebrate Pathology
Volume160
DOIs
Publication statusPublished - 1 Jan 2019
Externally publishedYes

Fingerprint

Helicoverpa armigera
RNA
cotton
virus
viruses
Picornavirales
RNA-directed RNA polymerase
RNA viruses
Nasonia vitripennis
Spodoptera exigua
eggs
virus transmission
food contamination
moths
moth
Drosophila
amino acid sequences
digestive system
Lepidoptera
genome

Keywords

  • Cotton bollworm
  • Covert virus
  • Helicoverpa armigera
  • Picorna-like viruses
  • Transmission strategy

Cite this

Yang, Xianming ; Xu, Pengjun ; Yuan, He ; Graham, Robert I. ; Wilson, Kenneth ; Wu, Kongming. / Discovery and characterization of a novel picorna-like RNA virus in the cotton bollworm Helicoverpa armigera. In: Journal of Invertebrate Pathology. 2019 ; Vol. 160. pp. 1-7.
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abstract = "We characterize a novel picorna-like virus, named Helicoverpa armigera Nora virus (HaNV), with a genome length of 11,200 nts, the sequence of which was isolated from the lepidopteran host cotton bollworm Helicoverpa armigera, using RNA-Seq. Phylogenetic analysis, using the putative amino acid sequence of the conserved RNA-dependent RNA polymerase (RdRp) domain, indicated that HaNV clustered with Spodoptera exigua Nora virus, Drosophila Nora virus and Nasonia vitripennis virus-3 with a high bootstrap value (100{\%}), which might indicate a new viral family within the order Picornavirales. HaNV was efficiently horizontally transmitted between hosts via contaminated food, and transmission was found to be dose-dependent (up to 100{\%} efficiency with 109 viral copy number/µl). HaNV was also found to be transmitted vertically from parent to offspring, mainly through transovum transmission (virus contamination on the surface of the eggs), but having a lower transmission efficiency (around 43{\%}). Infection distribution within the host was also investigated, with HaNV mainly found in only the gut of both adult moths and larvae (>90{\%}). Moreover, our results showed that HaNV appears not to be an overtly pathogenic virus to its host.",
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Discovery and characterization of a novel picorna-like RNA virus in the cotton bollworm Helicoverpa armigera. / Yang, Xianming; Xu, Pengjun; Yuan, He; Graham, Robert I.; Wilson, Kenneth; Wu, Kongming.

In: Journal of Invertebrate Pathology, Vol. 160, 01.01.2019, p. 1-7.

Research output: Contribution to journalJournal Article

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