Evaluation of entomopathogenic fungi as potential biological control agents of the dengue mosquito, aedes aegypti (diptera: Culicidae)

Jonathan M. Darbro, Robert I. Graham, Brian H. Kay, Peter A. Ryan, Matthew B. Thomas

Research output: Contribution to journalJournal Article

23 Citations (Scopus)

Abstract

Dengue is a global health concern. Growing insecticide resistance in the primary mosquito vector, Aedes aegypti, limits the effectiveness of vector control, so alternative tools are urgently needed. One approach is the use of biopesticides comprising entomopathogenic fungi, e.g., Beauveria bassiana and Metarhizium anisopliae. These fungi may decrease disease transmission by reducing mosquito vector longevity and also occur worldwide, although many isolates have not been tested for virulence against mosquitoes. Ninety-three isolates of entomopatho- genic fungi representing six species (B. bassiana, M. anisopliae, Isaria fumosoro- sea, I. farinosa, I. flavovirescens, and Lecanicillium spp.) were screened as potential biological control agents of Aedes aegypti. A hierarchical, multi-criteria experi- mental design was undertaken to find suitable isolates. Initial screening was performed via in vitro assays measuring radial growth and spore persistence, eliminating isolates with poor growth or viability on nutrient-rich substrate. Subsequent measurements of spore persistence revealed that only nine of 30 strains tested had half-lives exceeding 3 weeks. Ten isolates were chosen for in vivo bioassays against adult Ae. aegypti. From these assays, two Australian isolates of B. bassiana, FI-277 and FI-278, appeared to be most promising. Both isolates were shown to be virulent against Ae. aegypti at 20, 26, and 328C. Spreading spores manually onto substrate was found to be more efficacious than spraying. Ae. aegypti infected by manually-spread spores on cotton substrate were found to have an LT50 of 3.790.3 days. These characteristics suggest that FI-277 has promise as a dengue mosquito biocontrol agent, either alone or combined with conventional chemical insecticides.
Original languageEnglish
Pages (from-to)1027-1047
Number of pages21
JournalBiocontrol Science and Technology
Volume21
Issue number9
DOIs
Publication statusPublished - Sep 2011
Externally publishedYes

Fingerprint

dengue
entomopathogenic fungi
Aedes aegypti
biological control agents
Culicidae
Beauveria bassiana
spores
Metarhizium anisopliae
Isaria farinosa
Isaria fumosorosea
fungi
biopesticides
vector control
insecticide resistance
assays
disease transmission
spraying
half life
insecticides
virulence

Keywords

  • Aedes aegypti
  • Australia
  • Beauveria bassiana
  • Dengue
  • Metarhizium anisopliae
  • Mosquito

Cite this

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title = "Evaluation of entomopathogenic fungi as potential biological control agents of the dengue mosquito, aedes aegypti (diptera: Culicidae)",
abstract = "Dengue is a global health concern. Growing insecticide resistance in the primary mosquito vector, Aedes aegypti, limits the effectiveness of vector control, so alternative tools are urgently needed. One approach is the use of biopesticides comprising entomopathogenic fungi, e.g., Beauveria bassiana and Metarhizium anisopliae. These fungi may decrease disease transmission by reducing mosquito vector longevity and also occur worldwide, although many isolates have not been tested for virulence against mosquitoes. Ninety-three isolates of entomopatho- genic fungi representing six species (B. bassiana, M. anisopliae, Isaria fumosoro- sea, I. farinosa, I. flavovirescens, and Lecanicillium spp.) were screened as potential biological control agents of Aedes aegypti. A hierarchical, multi-criteria experi- mental design was undertaken to find suitable isolates. Initial screening was performed via in vitro assays measuring radial growth and spore persistence, eliminating isolates with poor growth or viability on nutrient-rich substrate. Subsequent measurements of spore persistence revealed that only nine of 30 strains tested had half-lives exceeding 3 weeks. Ten isolates were chosen for in vivo bioassays against adult Ae. aegypti. From these assays, two Australian isolates of B. bassiana, FI-277 and FI-278, appeared to be most promising. Both isolates were shown to be virulent against Ae. aegypti at 20, 26, and 328C. Spreading spores manually onto substrate was found to be more efficacious than spraying. Ae. aegypti infected by manually-spread spores on cotton substrate were found to have an LT50 of 3.790.3 days. These characteristics suggest that FI-277 has promise as a dengue mosquito biocontrol agent, either alone or combined with conventional chemical insecticides.",
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Evaluation of entomopathogenic fungi as potential biological control agents of the dengue mosquito, aedes aegypti (diptera: Culicidae). / Darbro, Jonathan M.; Graham, Robert I.; Kay, Brian H.; Ryan, Peter A.; Thomas, Matthew B.

In: Biocontrol Science and Technology, Vol. 21, No. 9, 09.2011, p. 1027-1047.

Research output: Contribution to journalJournal Article

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AU - Darbro, Jonathan M.

AU - Graham, Robert I.

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AU - Thomas, Matthew B.

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AB - Dengue is a global health concern. Growing insecticide resistance in the primary mosquito vector, Aedes aegypti, limits the effectiveness of vector control, so alternative tools are urgently needed. One approach is the use of biopesticides comprising entomopathogenic fungi, e.g., Beauveria bassiana and Metarhizium anisopliae. These fungi may decrease disease transmission by reducing mosquito vector longevity and also occur worldwide, although many isolates have not been tested for virulence against mosquitoes. Ninety-three isolates of entomopatho- genic fungi representing six species (B. bassiana, M. anisopliae, Isaria fumosoro- sea, I. farinosa, I. flavovirescens, and Lecanicillium spp.) were screened as potential biological control agents of Aedes aegypti. A hierarchical, multi-criteria experi- mental design was undertaken to find suitable isolates. Initial screening was performed via in vitro assays measuring radial growth and spore persistence, eliminating isolates with poor growth or viability on nutrient-rich substrate. Subsequent measurements of spore persistence revealed that only nine of 30 strains tested had half-lives exceeding 3 weeks. Ten isolates were chosen for in vivo bioassays against adult Ae. aegypti. From these assays, two Australian isolates of B. bassiana, FI-277 and FI-278, appeared to be most promising. Both isolates were shown to be virulent against Ae. aegypti at 20, 26, and 328C. Spreading spores manually onto substrate was found to be more efficacious than spraying. Ae. aegypti infected by manually-spread spores on cotton substrate were found to have an LT50 of 3.790.3 days. These characteristics suggest that FI-277 has promise as a dengue mosquito biocontrol agent, either alone or combined with conventional chemical insecticides.

KW - Aedes aegypti

KW - Australia

KW - Beauveria bassiana

KW - Dengue

KW - Metarhizium anisopliae

KW - Mosquito

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