Genetically variable nucleopolyhedroviruses isolated from spatially separate populations of the winter moth Operophtera brumata (Lepidoptera: Geometridae) in Orkney

Robert I. Graham, William I. Tyne, Robert D. Possee, Steven M. Sait, Rosemary S. Hails

Research output: Contribution to journalJournal Article

41 Citations (Scopus)

Abstract

Here we report a lepidopteran system in which a pathogen is both abundant and genotypically variable. Geographically separate populations of winter moth (Operophtera brumata L.) were sampled in heather habitats on the Orkney Isles to investigate the prevalence of a pathogen, O. brumata Nucleopolyhedrovirus (OpbuNPV), within the natural system. Virus was recorded in 11 of the 13 winter moth populations sampled, with two populations suffering mortality due to virus at levels of ≥50%. The virus genome from 200 single insect isolations was investigated for variation using restriction endonuclease digests. Twenty-six variants of OpbuNPV were detected using SalI. The polyhedrin gene of the virus was partially sequenced, allowing the relationship between the 26 variants to be portrayed as a cladogram. The phylogenetic relationship between OpbuNPV and other known baculovirus polyhedrin gene sequences was also established. The discovery of virus at such high prevalence is discussed with reference to occurrence and genetic variation of pathogens in other lepidopteran host populations. This study shows encouraging results for further studies into the role of pathogens in the regulation of host insect populations. © 2004 Elsevier Inc. All rights reserved.
Original languageEnglish
Pages (from-to)29-38
Number of pages10
JournalJournal of Invertebrate Pathology
Volume87
Issue number1
DOIs
Publication statusPublished - Sep 2004
Externally publishedYes

Fingerprint

Operophtera brumata
Geometridae
Baculoviridae
moth
pathogen
Lepidoptera
virus
pathogens
winter
viruses
insect
insects
gene
restriction endonucleases
genetic variation
genome
phylogenetics
nucleotide sequences
mortality
phylogeny

Keywords

  • Genetic variation
  • Nucleopolyhedrovirus
  • Operophtera brumata
  • Pathogen regulation
  • Polyhedrin gene

Cite this

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title = "Genetically variable nucleopolyhedroviruses isolated from spatially separate populations of the winter moth Operophtera brumata (Lepidoptera: Geometridae) in Orkney",
abstract = "Here we report a lepidopteran system in which a pathogen is both abundant and genotypically variable. Geographically separate populations of winter moth (Operophtera brumata L.) were sampled in heather habitats on the Orkney Isles to investigate the prevalence of a pathogen, O. brumata Nucleopolyhedrovirus (OpbuNPV), within the natural system. Virus was recorded in 11 of the 13 winter moth populations sampled, with two populations suffering mortality due to virus at levels of ≥50{\%}. The virus genome from 200 single insect isolations was investigated for variation using restriction endonuclease digests. Twenty-six variants of OpbuNPV were detected using SalI. The polyhedrin gene of the virus was partially sequenced, allowing the relationship between the 26 variants to be portrayed as a cladogram. The phylogenetic relationship between OpbuNPV and other known baculovirus polyhedrin gene sequences was also established. The discovery of virus at such high prevalence is discussed with reference to occurrence and genetic variation of pathogens in other lepidopteran host populations. This study shows encouraging results for further studies into the role of pathogens in the regulation of host insect populations. {\circledC} 2004 Elsevier Inc. All rights reserved.",
keywords = "Genetic variation, Nucleopolyhedrovirus, Operophtera brumata, Pathogen regulation, Polyhedrin gene",
author = "Graham, {Robert I.} and Tyne, {William I.} and Possee, {Robert D.} and Sait, {Steven M.} and Hails, {Rosemary S.}",
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Genetically variable nucleopolyhedroviruses isolated from spatially separate populations of the winter moth Operophtera brumata (Lepidoptera: Geometridae) in Orkney. / Graham, Robert I.; Tyne, William I.; Possee, Robert D.; Sait, Steven M.; Hails, Rosemary S.

In: Journal of Invertebrate Pathology, Vol. 87, No. 1, 09.2004, p. 29-38.

Research output: Contribution to journalJournal Article

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T1 - Genetically variable nucleopolyhedroviruses isolated from spatially separate populations of the winter moth Operophtera brumata (Lepidoptera: Geometridae) in Orkney

AU - Graham, Robert I.

AU - Tyne, William I.

AU - Possee, Robert D.

AU - Sait, Steven M.

AU - Hails, Rosemary S.

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N2 - Here we report a lepidopteran system in which a pathogen is both abundant and genotypically variable. Geographically separate populations of winter moth (Operophtera brumata L.) were sampled in heather habitats on the Orkney Isles to investigate the prevalence of a pathogen, O. brumata Nucleopolyhedrovirus (OpbuNPV), within the natural system. Virus was recorded in 11 of the 13 winter moth populations sampled, with two populations suffering mortality due to virus at levels of ≥50%. The virus genome from 200 single insect isolations was investigated for variation using restriction endonuclease digests. Twenty-six variants of OpbuNPV were detected using SalI. The polyhedrin gene of the virus was partially sequenced, allowing the relationship between the 26 variants to be portrayed as a cladogram. The phylogenetic relationship between OpbuNPV and other known baculovirus polyhedrin gene sequences was also established. The discovery of virus at such high prevalence is discussed with reference to occurrence and genetic variation of pathogens in other lepidopteran host populations. This study shows encouraging results for further studies into the role of pathogens in the regulation of host insect populations. © 2004 Elsevier Inc. All rights reserved.

AB - Here we report a lepidopteran system in which a pathogen is both abundant and genotypically variable. Geographically separate populations of winter moth (Operophtera brumata L.) were sampled in heather habitats on the Orkney Isles to investigate the prevalence of a pathogen, O. brumata Nucleopolyhedrovirus (OpbuNPV), within the natural system. Virus was recorded in 11 of the 13 winter moth populations sampled, with two populations suffering mortality due to virus at levels of ≥50%. The virus genome from 200 single insect isolations was investigated for variation using restriction endonuclease digests. Twenty-six variants of OpbuNPV were detected using SalI. The polyhedrin gene of the virus was partially sequenced, allowing the relationship between the 26 variants to be portrayed as a cladogram. The phylogenetic relationship between OpbuNPV and other known baculovirus polyhedrin gene sequences was also established. The discovery of virus at such high prevalence is discussed with reference to occurrence and genetic variation of pathogens in other lepidopteran host populations. This study shows encouraging results for further studies into the role of pathogens in the regulation of host insect populations. © 2004 Elsevier Inc. All rights reserved.

KW - Genetic variation

KW - Nucleopolyhedrovirus

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