Genome evolution and the emergence of pathogenicity in avian Escherichia coli

Leonardos Mageiros, Guillaume Méric, Sion C. Bayliss, Johan Pensar, Ben Pascoe, Evangelos Mourkas, Jessica K. Calland, Koji Yahara, Susan Murray, Thomas S. Wilkinson Wilkinson, Lisa Williams, Matthew D. Hitchings, Jonathan Porter, Kirsty Kemmett, Edward J. Feil, Keith A. Jolley, Nicola J. Williams, Jukka Corander, Samuel K. Sheppard

Research output: Contribution to journalJournal Articlepeer-review

Abstract

Chickens are the most common birds on Earth and colibacillosis is among the most common diseases affecting them. This major threat to animal welfare and safe sustainable food production is difficult to combat because the etiological agent, avian pathogenic Escherichia coli (APEC), emerges from ubiquitous commensal gut bacteria, with no single virulence gene present in all disease-causing isolates. Here, we address the underlying evolutionary mechanisms of extraintestinal spread and systemic infection in poultry. Combining population scale comparative genomics and pangenome-wide association studies, we compare E. coli from commensal carriage and systemic infections. We identify phylogroup-specific and species-wide genetic elements that are enriched in APEC, including pathogenicity-associated variation in 143 genes that have diverse functions, including genes involved in metabolism, lipopolysaccharide synthesis, heat shock response, antimicrobial resistance and toxicity. We find that horizontal gene transfer spreads pathogenicity elements, allowing divergent clones to cause infection. Finally, a Random Forest model prediction of disease status (carriage vs. disease) identifies pathogenic strains in the emergent ST-117 poultry-associated lineage with 73% accuracy, demonstrating the potential for early identification of emergent APEC in healthy flocks.
Original languageEnglish
Article number765 (2021)
JournalNature Communications
Volume12
DOIs
Publication statusPublished - 3 Feb 2021
Externally publishedYes

Keywords

  • Animals
  • Chickens
  • Escherichia coli Infections/diagnosis
  • Escherichia coli/classification
  • Evolution, Molecular
  • Genes, Bacterial
  • Genetic Variation
  • Genome, Bacterial/genetics
  • Genome-Wide Association Study/methods
  • Genotype
  • Humans
  • Phylogeny
  • Poultry Diseases/diagnosis
  • Virulence/genetics

Fingerprint

Dive into the research topics of 'Genome evolution and the emergence of pathogenicity in avian Escherichia coli'. Together they form a unique fingerprint.

Cite this