mcr-1 and mcr-2 (mcr-6.1) variant genes identified in Moraxella species isolated from pigs in Great Britain from 2014 to 2015

Manal AbuOun, Emma J Stubberfield, Nick A Duggett, Miranda Kirchner, Luisa Dormer, Javier Nunez-Garcia, Luke P Randall , Fabrizio Lemma, Derrick W Cook , Christopher Teale, Richard P Smith, Muna F Anjum

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Abstract

Objectives
To determine the occurrence of mcr-1 and mcr-2 genes in Gram-negative bacteria isolated from healthy pigs in Great Britain.
Methods
Gram-negative bacteria (n = 657) isolated from pigs between 2014 and 2015 were examined by WGS.
Results
Variants of mcr-1 and mcr-2 were identified in Moraxella spp. isolated from pooled caecal contents of healthy pigs at slaughter collected from six farms in Great Britain. Other bacteria, including Escherichia coli from the same farms, were not detected harbouring mcr-1 or mcr-2. A Moraxella porci-like isolate, MSG13-C03, harboured MCR-1.10 with 98.7% identity to MCR-1, and we describe the first detection of mcr-6.1, found on the chromosome of Moraxella pluranimalium, with 87.9% identity to MCR-2 from E. coli. The Moraxella isolates had colistin MICs of 1–2 mg/L. No intact insertion elements were identified in either MSG13-C03 or MSG47-C17, although MSG13-C03 harboured the conserved nucleotides abutting the ISApl1 composite transposon found in E. coli plasmids and the intervening ∼2.6 kb fragment showed 97% identity. Six Moraxella osloensis isolates were positive for phosphoethanolamine transferase (EptA). They shared 62%–64.5% identity to MCR-1 and MCR-2, with colistin MICs from 2 to 4 mg/L. Phylogenetic analysis indicated that MCR and EptA have evolved from a common ancestor. In addition to mcr, the β-lactamase gene, blaBRO-1, was found in both isolates, whilst the tetracycline resistance gene, tetL, was found in MSG47-C17.
Conclusions
Our results add further evidence for the mobilization of the mcr-pap2 unit from Moraxella via composite transposons leading to its global dissemination. The presence of mcr-pap2 from recent Moraxella isolates indicates they may comprise a reservoir for mcr.
Original languageEnglish
Pages (from-to)2745-2749
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Volume73
Issue number10
DOIs
Publication statusPublished - 11 Aug 2017
Externally publishedYes

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Moraxella
Swine
Genes
Colistin
Escherichia coli
Bacteria
Tetracycline Resistance
Transferases
United Kingdom
Gram-Negative Bacteria
Plasmids
Nucleotides
Chromosomes

Cite this

AbuOun, Manal ; Stubberfield, Emma J ; Duggett, Nick A ; Kirchner, Miranda ; Dormer, Luisa ; Nunez-Garcia, Javier ; Randall , Luke P ; Lemma, Fabrizio ; Cook , Derrick W ; Teale, Christopher ; Smith, Richard P ; Anjum, Muna F . / mcr-1 and mcr-2 (mcr-6.1) variant genes identified in Moraxella species isolated from pigs in Great Britain from 2014 to 2015. In: Journal of Antimicrobial Chemotherapy. 2017 ; Vol. 73, No. 10. pp. 2745-2749.
@article{f8b078c1046c4c7a9cc0f21abbcb64ba,
title = "mcr-1 and mcr-2 (mcr-6.1) variant genes identified in Moraxella species isolated from pigs in Great Britain from 2014 to 2015",
abstract = "ObjectivesTo determine the occurrence of mcr-1 and mcr-2 genes in Gram-negative bacteria isolated from healthy pigs in Great Britain.MethodsGram-negative bacteria (n = 657) isolated from pigs between 2014 and 2015 were examined by WGS.ResultsVariants of mcr-1 and mcr-2 were identified in Moraxella spp. isolated from pooled caecal contents of healthy pigs at slaughter collected from six farms in Great Britain. Other bacteria, including Escherichia coli from the same farms, were not detected harbouring mcr-1 or mcr-2. A Moraxella porci-like isolate, MSG13-C03, harboured MCR-1.10 with 98.7{\%} identity to MCR-1, and we describe the first detection of mcr-6.1, found on the chromosome of Moraxella pluranimalium, with 87.9{\%} identity to MCR-2 from E. coli. The Moraxella isolates had colistin MICs of 1–2 mg/L. No intact insertion elements were identified in either MSG13-C03 or MSG47-C17, although MSG13-C03 harboured the conserved nucleotides abutting the ISApl1 composite transposon found in E. coli plasmids and the intervening ∼2.6 kb fragment showed 97{\%} identity. Six Moraxella osloensis isolates were positive for phosphoethanolamine transferase (EptA). They shared 62{\%}–64.5{\%} identity to MCR-1 and MCR-2, with colistin MICs from 2 to 4 mg/L. Phylogenetic analysis indicated that MCR and EptA have evolved from a common ancestor. In addition to mcr, the β-lactamase gene, blaBRO-1, was found in both isolates, whilst the tetracycline resistance gene, tetL, was found in MSG47-C17.ConclusionsOur results add further evidence for the mobilization of the mcr-pap2 unit from Moraxella via composite transposons leading to its global dissemination. The presence of mcr-pap2 from recent Moraxella isolates indicates they may comprise a reservoir for mcr.",
author = "Manal AbuOun and Stubberfield, {Emma J} and Duggett, {Nick A} and Miranda Kirchner and Luisa Dormer and Javier Nunez-Garcia and Randall, {Luke P} and Fabrizio Lemma and Cook, {Derrick W} and Christopher Teale and Smith, {Richard P} and Anjum, {Muna F}",
year = "2017",
month = "8",
day = "11",
doi = "10.1093/jac/dkx286",
language = "English",
volume = "73",
pages = "2745--2749",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "10",

}

AbuOun, M, Stubberfield, EJ, Duggett, NA, Kirchner, M, Dormer, L, Nunez-Garcia, J, Randall , LP, Lemma, F, Cook , DW, Teale, C, Smith, RP & Anjum, MF 2017, 'mcr-1 and mcr-2 (mcr-6.1) variant genes identified in Moraxella species isolated from pigs in Great Britain from 2014 to 2015', Journal of Antimicrobial Chemotherapy, vol. 73, no. 10, pp. 2745-2749. https://doi.org/10.1093/jac/dkx286

mcr-1 and mcr-2 (mcr-6.1) variant genes identified in Moraxella species isolated from pigs in Great Britain from 2014 to 2015. / AbuOun, Manal; Stubberfield, Emma J; Duggett, Nick A; Kirchner, Miranda; Dormer, Luisa; Nunez-Garcia, Javier; Randall , Luke P ; Lemma, Fabrizio ; Cook , Derrick W; Teale, Christopher; Smith, Richard P; Anjum, Muna F .

In: Journal of Antimicrobial Chemotherapy, Vol. 73, No. 10, 11.08.2017, p. 2745-2749.

Research output: Contribution to journalJournal Article

TY - JOUR

T1 - mcr-1 and mcr-2 (mcr-6.1) variant genes identified in Moraxella species isolated from pigs in Great Britain from 2014 to 2015

AU - AbuOun, Manal

AU - Stubberfield, Emma J

AU - Duggett, Nick A

AU - Kirchner, Miranda

AU - Dormer, Luisa

AU - Nunez-Garcia, Javier

AU - Randall , Luke P

AU - Lemma, Fabrizio

AU - Cook , Derrick W

AU - Teale, Christopher

AU - Smith, Richard P

AU - Anjum, Muna F

PY - 2017/8/11

Y1 - 2017/8/11

N2 - ObjectivesTo determine the occurrence of mcr-1 and mcr-2 genes in Gram-negative bacteria isolated from healthy pigs in Great Britain.MethodsGram-negative bacteria (n = 657) isolated from pigs between 2014 and 2015 were examined by WGS.ResultsVariants of mcr-1 and mcr-2 were identified in Moraxella spp. isolated from pooled caecal contents of healthy pigs at slaughter collected from six farms in Great Britain. Other bacteria, including Escherichia coli from the same farms, were not detected harbouring mcr-1 or mcr-2. A Moraxella porci-like isolate, MSG13-C03, harboured MCR-1.10 with 98.7% identity to MCR-1, and we describe the first detection of mcr-6.1, found on the chromosome of Moraxella pluranimalium, with 87.9% identity to MCR-2 from E. coli. The Moraxella isolates had colistin MICs of 1–2 mg/L. No intact insertion elements were identified in either MSG13-C03 or MSG47-C17, although MSG13-C03 harboured the conserved nucleotides abutting the ISApl1 composite transposon found in E. coli plasmids and the intervening ∼2.6 kb fragment showed 97% identity. Six Moraxella osloensis isolates were positive for phosphoethanolamine transferase (EptA). They shared 62%–64.5% identity to MCR-1 and MCR-2, with colistin MICs from 2 to 4 mg/L. Phylogenetic analysis indicated that MCR and EptA have evolved from a common ancestor. In addition to mcr, the β-lactamase gene, blaBRO-1, was found in both isolates, whilst the tetracycline resistance gene, tetL, was found in MSG47-C17.ConclusionsOur results add further evidence for the mobilization of the mcr-pap2 unit from Moraxella via composite transposons leading to its global dissemination. The presence of mcr-pap2 from recent Moraxella isolates indicates they may comprise a reservoir for mcr.

AB - ObjectivesTo determine the occurrence of mcr-1 and mcr-2 genes in Gram-negative bacteria isolated from healthy pigs in Great Britain.MethodsGram-negative bacteria (n = 657) isolated from pigs between 2014 and 2015 were examined by WGS.ResultsVariants of mcr-1 and mcr-2 were identified in Moraxella spp. isolated from pooled caecal contents of healthy pigs at slaughter collected from six farms in Great Britain. Other bacteria, including Escherichia coli from the same farms, were not detected harbouring mcr-1 or mcr-2. A Moraxella porci-like isolate, MSG13-C03, harboured MCR-1.10 with 98.7% identity to MCR-1, and we describe the first detection of mcr-6.1, found on the chromosome of Moraxella pluranimalium, with 87.9% identity to MCR-2 from E. coli. The Moraxella isolates had colistin MICs of 1–2 mg/L. No intact insertion elements were identified in either MSG13-C03 or MSG47-C17, although MSG13-C03 harboured the conserved nucleotides abutting the ISApl1 composite transposon found in E. coli plasmids and the intervening ∼2.6 kb fragment showed 97% identity. Six Moraxella osloensis isolates were positive for phosphoethanolamine transferase (EptA). They shared 62%–64.5% identity to MCR-1 and MCR-2, with colistin MICs from 2 to 4 mg/L. Phylogenetic analysis indicated that MCR and EptA have evolved from a common ancestor. In addition to mcr, the β-lactamase gene, blaBRO-1, was found in both isolates, whilst the tetracycline resistance gene, tetL, was found in MSG47-C17.ConclusionsOur results add further evidence for the mobilization of the mcr-pap2 unit from Moraxella via composite transposons leading to its global dissemination. The presence of mcr-pap2 from recent Moraxella isolates indicates they may comprise a reservoir for mcr.

U2 - 10.1093/jac/dkx286

DO - 10.1093/jac/dkx286

M3 - Journal Article

VL - 73

SP - 2745

EP - 2749

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 10

ER -