Molecular characterisation of a cypovirus isolated from the western spruce budworm Choristoneura occidentalis

Robert I. Graham, Benoit Morin, Renée Lapointe, Vincent G. Nealis, Christopher J. Lucarotti

Research output: Contribution to journalJournal Article

Abstract

A novel cypovirus, assigned CoCPV, was isolated from natural populations of the western spruce budworm, Choristoneura occidentalis. The complete nucleotide sequences of genomic segments S2-S5 and S7-S10 were determined. Each segment contained a single open reading frame. Conserved motifs 5' (AGUUU......UUUGUGC) 3' were found at the ends of each segment. Analysis of S2, which encoded a putative RNA-dependent RNA polymerase protein, confirmed CoCPV belonged to the genus Cypovirus within the family Reoviridae. Further phylogenetic analysis using S10 (the polyhedrin gene) aligned this virus with species type-16, closely related to a cypovirus isolated from C. fumiferana.
Original languageEnglish
Pages (from-to)1759-1763
Number of pages5
JournalArchives of Virology
Volume153
Issue number9
DOIs
Publication statusPublished - Sep 2008
Externally publishedYes

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Reoviridae
S 10
ethyl-2-methylthio-4-methyl-5-pyrimidine carboxylate
RNA Replicase
Open Reading Frames
Viruses
Population
Genes
Proteins

Cite this

Graham, Robert I. ; Morin, Benoit ; Lapointe, Renée ; Nealis, Vincent G. ; Lucarotti, Christopher J. / Molecular characterisation of a cypovirus isolated from the western spruce budworm Choristoneura occidentalis. In: Archives of Virology. 2008 ; Vol. 153, No. 9. pp. 1759-1763.
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Molecular characterisation of a cypovirus isolated from the western spruce budworm Choristoneura occidentalis. / Graham, Robert I.; Morin, Benoit; Lapointe, Renée; Nealis, Vincent G.; Lucarotti, Christopher J.

In: Archives of Virology, Vol. 153, No. 9, 09.2008, p. 1759-1763.

Research output: Contribution to journalJournal Article

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AU - Lucarotti, Christopher J.

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