New insights into the phylogeny of Entamoeba species provided by analysis of four new small-subunit rRNA genes

C. Graham Clark, Farrokh Kaffashian, Blessing Tawari, Jeffrey J. Windsor, Anke Twigg-Flesner, Mina C G Davies-Morel, Joerg Blessmann, Frank Ebert, Babett Peschel, An Le Van, Colin J. Jackson, Lorna Macfarlane, Egbert Tannich

Research output: Contribution to journalJournal Article

54 Citations (Scopus)

Abstract

Sequences of small-subunit rRNA genes have been obtained for four new isolates of Entamoeba. Phylogenetic analyses give new insights into the evolution of these organisms. A novel Entamoeba from pigs in Vietnam that produces uninucleate cysts proved to be unrelated to other uninucleated cyst-producing species. Revival of the name Entamoeba suis for this organism is proposed. Instead of being related to Entamoeba polecki, it shares a recent common ancestor with the non-encysting Entamoeba gingivalis in a lineage that is basal to the tetranucleate cyst-producing clade. This suggests that species producing cysts with four nuclei are descended from an ancestor that produced cysts with a single nucleus. An Entamoeba from a horse was isolated in culture. No cysts were observed in the original stool sample but the sequence is placed unequivocally within the clade of tetranucleate cyst-producing species with no other sequences being specifically related. Revival of the name Entamoeba equi for this organism is proposed. The Entamoeba ecuadoriensis sequence was found to be the most closely related to Entamoeba histolytica and Entamoeba dispar, as predicted, despite the organism having been an environmental isolate originally assigned to Entamoeba moshkovskii. Finally, a partial E. polecki gene sequence from a pig proved to be virtually identical to that of Entamoeba struthionis from an ostrich, suggesting that the latter name is a synonym.
Original languageEnglish
Pages (from-to)2235-2239
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume56
Issue number9
DOIs
Publication statusPublished - Sep 2006
Externally publishedYes

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Entamoeba
Phylogeny
cyst
rRNA Genes
phylogeny
ribosomal RNA
gene
Cysts
genes
pig
Names
organisms
ancestry
common ancestry
horse
analysis
Swine
Entamoeba histolytica
Struthioniformes
swine

Cite this

Clark, C. Graham ; Kaffashian, Farrokh ; Tawari, Blessing ; Windsor, Jeffrey J. ; Twigg-Flesner, Anke ; Davies-Morel, Mina C G ; Blessmann, Joerg ; Ebert, Frank ; Peschel, Babett ; Le Van, An ; Jackson, Colin J. ; Macfarlane, Lorna ; Tannich, Egbert. / New insights into the phylogeny of Entamoeba species provided by analysis of four new small-subunit rRNA genes. In: International Journal of Systematic and Evolutionary Microbiology. 2006 ; Vol. 56, No. 9. pp. 2235-2239.
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title = "New insights into the phylogeny of Entamoeba species provided by analysis of four new small-subunit rRNA genes",
abstract = "Sequences of small-subunit rRNA genes have been obtained for four new isolates of Entamoeba. Phylogenetic analyses give new insights into the evolution of these organisms. A novel Entamoeba from pigs in Vietnam that produces uninucleate cysts proved to be unrelated to other uninucleated cyst-producing species. Revival of the name Entamoeba suis for this organism is proposed. Instead of being related to Entamoeba polecki, it shares a recent common ancestor with the non-encysting Entamoeba gingivalis in a lineage that is basal to the tetranucleate cyst-producing clade. This suggests that species producing cysts with four nuclei are descended from an ancestor that produced cysts with a single nucleus. An Entamoeba from a horse was isolated in culture. No cysts were observed in the original stool sample but the sequence is placed unequivocally within the clade of tetranucleate cyst-producing species with no other sequences being specifically related. Revival of the name Entamoeba equi for this organism is proposed. The Entamoeba ecuadoriensis sequence was found to be the most closely related to Entamoeba histolytica and Entamoeba dispar, as predicted, despite the organism having been an environmental isolate originally assigned to Entamoeba moshkovskii. Finally, a partial E. polecki gene sequence from a pig proved to be virtually identical to that of Entamoeba struthionis from an ostrich, suggesting that the latter name is a synonym.",
author = "Clark, {C. Graham} and Farrokh Kaffashian and Blessing Tawari and Windsor, {Jeffrey J.} and Anke Twigg-Flesner and Davies-Morel, {Mina C G} and Joerg Blessmann and Frank Ebert and Babett Peschel and {Le Van}, An and Jackson, {Colin J.} and Lorna Macfarlane and Egbert Tannich",
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Clark, CG, Kaffashian, F, Tawari, B, Windsor, JJ, Twigg-Flesner, A, Davies-Morel, MCG, Blessmann, J, Ebert, F, Peschel, B, Le Van, A, Jackson, CJ, Macfarlane, L & Tannich, E 2006, 'New insights into the phylogeny of Entamoeba species provided by analysis of four new small-subunit rRNA genes', International Journal of Systematic and Evolutionary Microbiology, vol. 56, no. 9, pp. 2235-2239. https://doi.org/10.1099/ijs.0.64208-0

New insights into the phylogeny of Entamoeba species provided by analysis of four new small-subunit rRNA genes. / Clark, C. Graham; Kaffashian, Farrokh; Tawari, Blessing; Windsor, Jeffrey J.; Twigg-Flesner, Anke; Davies-Morel, Mina C G; Blessmann, Joerg; Ebert, Frank; Peschel, Babett; Le Van, An; Jackson, Colin J.; Macfarlane, Lorna; Tannich, Egbert.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 56, No. 9, 09.2006, p. 2235-2239.

Research output: Contribution to journalJournal Article

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T1 - New insights into the phylogeny of Entamoeba species provided by analysis of four new small-subunit rRNA genes

AU - Clark, C. Graham

AU - Kaffashian, Farrokh

AU - Tawari, Blessing

AU - Windsor, Jeffrey J.

AU - Twigg-Flesner, Anke

AU - Davies-Morel, Mina C G

AU - Blessmann, Joerg

AU - Ebert, Frank

AU - Peschel, Babett

AU - Le Van, An

AU - Jackson, Colin J.

AU - Macfarlane, Lorna

AU - Tannich, Egbert

PY - 2006/9

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N2 - Sequences of small-subunit rRNA genes have been obtained for four new isolates of Entamoeba. Phylogenetic analyses give new insights into the evolution of these organisms. A novel Entamoeba from pigs in Vietnam that produces uninucleate cysts proved to be unrelated to other uninucleated cyst-producing species. Revival of the name Entamoeba suis for this organism is proposed. Instead of being related to Entamoeba polecki, it shares a recent common ancestor with the non-encysting Entamoeba gingivalis in a lineage that is basal to the tetranucleate cyst-producing clade. This suggests that species producing cysts with four nuclei are descended from an ancestor that produced cysts with a single nucleus. An Entamoeba from a horse was isolated in culture. No cysts were observed in the original stool sample but the sequence is placed unequivocally within the clade of tetranucleate cyst-producing species with no other sequences being specifically related. Revival of the name Entamoeba equi for this organism is proposed. The Entamoeba ecuadoriensis sequence was found to be the most closely related to Entamoeba histolytica and Entamoeba dispar, as predicted, despite the organism having been an environmental isolate originally assigned to Entamoeba moshkovskii. Finally, a partial E. polecki gene sequence from a pig proved to be virtually identical to that of Entamoeba struthionis from an ostrich, suggesting that the latter name is a synonym.

AB - Sequences of small-subunit rRNA genes have been obtained for four new isolates of Entamoeba. Phylogenetic analyses give new insights into the evolution of these organisms. A novel Entamoeba from pigs in Vietnam that produces uninucleate cysts proved to be unrelated to other uninucleated cyst-producing species. Revival of the name Entamoeba suis for this organism is proposed. Instead of being related to Entamoeba polecki, it shares a recent common ancestor with the non-encysting Entamoeba gingivalis in a lineage that is basal to the tetranucleate cyst-producing clade. This suggests that species producing cysts with four nuclei are descended from an ancestor that produced cysts with a single nucleus. An Entamoeba from a horse was isolated in culture. No cysts were observed in the original stool sample but the sequence is placed unequivocally within the clade of tetranucleate cyst-producing species with no other sequences being specifically related. Revival of the name Entamoeba equi for this organism is proposed. The Entamoeba ecuadoriensis sequence was found to be the most closely related to Entamoeba histolytica and Entamoeba dispar, as predicted, despite the organism having been an environmental isolate originally assigned to Entamoeba moshkovskii. Finally, a partial E. polecki gene sequence from a pig proved to be virtually identical to that of Entamoeba struthionis from an ostrich, suggesting that the latter name is a synonym.

U2 - 10.1099/ijs.0.64208-0

DO - 10.1099/ijs.0.64208-0

M3 - Journal Article

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SP - 2235

EP - 2239

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 9

ER -