Preliminary examination of gut bacteria from Neodiprion abietis (Hymenoptera: Diprionidae) larvae

Beatrix Whittome, Robert Graham, David Levin

Research output: Contribution to journalJournal Article

Abstract

J. ent. Soc. Ont. 138: 49–63 The gut microbiotas of insects are important for many processes, including digestion, nitrogen fixation, and nutrient recycling. Bacterial 16S ribosomal DNA (rDNA) extracted from excised Neodiprion abietis larval guts was amplified using PCR. Two combinations of primers produced six fragments that were separated using Denaturing Gradient Gel Electrophoresis (DGGE). The DNA fragments were sequenced directly. BLAST-n analysis and comparison-rank searches, using the Ribosomal Database Project II, revealed four predicted bacterial species, one that had similarity to Alphaproteobacteria and three that aligned with Gammaproteobacteria. Phylogenetic analysis by maximum parsimony and neighbour joining confirmed these findings and suggest that Rahnella, Yersinia, Enterobacter, and a Caulobacter-like species inhabit the N. abietis larval gut.
Original languageEnglish
Pages (from-to)49-63
Number of pages15
JournalJournal of the Entomological Society of Ontario
Volume138
Publication statusPublished - 2007
Externally publishedYes

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Neodiprion abietis
Diprionidae
Rahnella
Caulobacter
digestive system
Hymenoptera
Yersinia
Enterobacter
larvae
alpha-Proteobacteria
gamma-Proteobacteria
bacteria
denaturing gradient gel electrophoresis
intestinal microorganisms
nitrogen fixation
ribosomal DNA
recycling
digestion
insects
phylogeny

Cite this

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abstract = "J. ent. Soc. Ont. 138: 49–63 The gut microbiotas of insects are important for many processes, including digestion, nitrogen fixation, and nutrient recycling. Bacterial 16S ribosomal DNA (rDNA) extracted from excised Neodiprion abietis larval guts was amplified using PCR. Two combinations of primers produced six fragments that were separated using Denaturing Gradient Gel Electrophoresis (DGGE). The DNA fragments were sequenced directly. BLAST-n analysis and comparison-rank searches, using the Ribosomal Database Project II, revealed four predicted bacterial species, one that had similarity to Alphaproteobacteria and three that aligned with Gammaproteobacteria. Phylogenetic analysis by maximum parsimony and neighbour joining confirmed these findings and suggest that Rahnella, Yersinia, Enterobacter, and a Caulobacter-like species inhabit the N. abietis larval gut.",
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Preliminary examination of gut bacteria from Neodiprion abietis (Hymenoptera: Diprionidae) larvae. / Whittome, Beatrix; Graham, Robert; Levin, David.

In: Journal of the Entomological Society of Ontario, Vol. 138, 2007, p. 49-63.

Research output: Contribution to journalJournal Article

TY - JOUR

T1 - Preliminary examination of gut bacteria from Neodiprion abietis (Hymenoptera: Diprionidae) larvae

AU - Whittome, Beatrix

AU - Graham, Robert

AU - Levin, David

PY - 2007

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N2 - J. ent. Soc. Ont. 138: 49–63 The gut microbiotas of insects are important for many processes, including digestion, nitrogen fixation, and nutrient recycling. Bacterial 16S ribosomal DNA (rDNA) extracted from excised Neodiprion abietis larval guts was amplified using PCR. Two combinations of primers produced six fragments that were separated using Denaturing Gradient Gel Electrophoresis (DGGE). The DNA fragments were sequenced directly. BLAST-n analysis and comparison-rank searches, using the Ribosomal Database Project II, revealed four predicted bacterial species, one that had similarity to Alphaproteobacteria and three that aligned with Gammaproteobacteria. Phylogenetic analysis by maximum parsimony and neighbour joining confirmed these findings and suggest that Rahnella, Yersinia, Enterobacter, and a Caulobacter-like species inhabit the N. abietis larval gut.

AB - J. ent. Soc. Ont. 138: 49–63 The gut microbiotas of insects are important for many processes, including digestion, nitrogen fixation, and nutrient recycling. Bacterial 16S ribosomal DNA (rDNA) extracted from excised Neodiprion abietis larval guts was amplified using PCR. Two combinations of primers produced six fragments that were separated using Denaturing Gradient Gel Electrophoresis (DGGE). The DNA fragments were sequenced directly. BLAST-n analysis and comparison-rank searches, using the Ribosomal Database Project II, revealed four predicted bacterial species, one that had similarity to Alphaproteobacteria and three that aligned with Gammaproteobacteria. Phylogenetic analysis by maximum parsimony and neighbour joining confirmed these findings and suggest that Rahnella, Yersinia, Enterobacter, and a Caulobacter-like species inhabit the N. abietis larval gut.

M3 - Journal Article

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EP - 63

JO - Journal of the Entomological Society of Ontario

JF - Journal of the Entomological Society of Ontario

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