Sequence analysis of a reovirus isolated from the winter moth Operophtera brumata (Lepidoptera: Geometridae) and its parasitoid wasp Phobocampe tempestiva (Hymenoptera: Ichneumonidae)

Robert I. Graham, Shujing Rao, Steven M. Sait, Houssam Attoui, Peter P.C. Mertens, Rosemary S. Hails, Robert D. Possee

Research output: Contribution to journalJournal Article

Abstract

A reovirus was isolated from Operophtera brumata (ObRV) and its parasitoid wasp Phobocampe tempestiva. Each of the 10 dsRNA genome segments of ObRV was sequenced and shown to contain a single open reading frame (ORF). Conserved motifs ([+ve] 5′-AAATAAA ... G/TAGGTT-3′) were found at the termini of each segment, with the exception of Seg-6 and Seg-8, where the 5′ termini were 5′-AACAAA...-3′. The putative proteins encoded by each segment were compared with those of other members of the family Reoviridae. Phylogenetic comparisons to published sequences for the RNA-dependent RNA polymerase genes from other reoviruses indicated that ObRV is most closely related to members of the genus Cypovirus. However, unlike the cypoviruses, ObRV has a double-layered capsid structure. When the protein encoded by ObRV Seg-10 was expressed (by inserting the open reading frame into a baculovirus expression vector) no 'occlusion bodies' were observed in the recombinant baculovirus infected insect cell cultures. This suggests that unlike the cypoviruses, Seg-10 of ObRV does not contain a polyhedrin gene. Further phylogenetic comparisons also identified relationships between Seg-2 and Seg-10 of ObRV, and genes of Diadromus pulchellus Idnoreovirus 1 (DpIRV1), suggesting that ObRV represents a new species from the genus Idnoreovirus. © 2008 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)42-47
Number of pages6
JournalVirus Research
Volume135
Issue number1
DOIs
Publication statusPublished - Jul 2008
Externally publishedYes

Fingerprint

Reoviridae
Wasps
Hymenoptera
Lepidoptera
Moths
Sequence Analysis
Baculoviridae
Open Reading Frames
Genes
RNA Replicase
Capsid
Insects
Proteins
Cell Culture Techniques
Genome

Keywords

  • Operophtera brumata
  • Protein expression
  • Reovirus
  • Winter moth

Cite this

Graham, Robert I. ; Rao, Shujing ; Sait, Steven M. ; Attoui, Houssam ; Mertens, Peter P.C. ; Hails, Rosemary S. ; Possee, Robert D. / Sequence analysis of a reovirus isolated from the winter moth Operophtera brumata (Lepidoptera: Geometridae) and its parasitoid wasp Phobocampe tempestiva (Hymenoptera: Ichneumonidae). In: Virus Research. 2008 ; Vol. 135, No. 1. pp. 42-47.
@article{ea3dd570aa66403eabebe776a6cba924,
title = "Sequence analysis of a reovirus isolated from the winter moth Operophtera brumata (Lepidoptera: Geometridae) and its parasitoid wasp Phobocampe tempestiva (Hymenoptera: Ichneumonidae)",
abstract = "A reovirus was isolated from Operophtera brumata (ObRV) and its parasitoid wasp Phobocampe tempestiva. Each of the 10 dsRNA genome segments of ObRV was sequenced and shown to contain a single open reading frame (ORF). Conserved motifs ([+ve] 5′-AAATAAA ... G/TAGGTT-3′) were found at the termini of each segment, with the exception of Seg-6 and Seg-8, where the 5′ termini were 5′-AACAAA...-3′. The putative proteins encoded by each segment were compared with those of other members of the family Reoviridae. Phylogenetic comparisons to published sequences for the RNA-dependent RNA polymerase genes from other reoviruses indicated that ObRV is most closely related to members of the genus Cypovirus. However, unlike the cypoviruses, ObRV has a double-layered capsid structure. When the protein encoded by ObRV Seg-10 was expressed (by inserting the open reading frame into a baculovirus expression vector) no 'occlusion bodies' were observed in the recombinant baculovirus infected insect cell cultures. This suggests that unlike the cypoviruses, Seg-10 of ObRV does not contain a polyhedrin gene. Further phylogenetic comparisons also identified relationships between Seg-2 and Seg-10 of ObRV, and genes of Diadromus pulchellus Idnoreovirus 1 (DpIRV1), suggesting that ObRV represents a new species from the genus Idnoreovirus. {\circledC} 2008 Elsevier B.V. All rights reserved.",
keywords = "Operophtera brumata, Protein expression, Reovirus, Winter moth",
author = "Graham, {Robert I.} and Shujing Rao and Sait, {Steven M.} and Houssam Attoui and Mertens, {Peter P.C.} and Hails, {Rosemary S.} and Possee, {Robert D.}",
year = "2008",
month = "7",
doi = "10.1016/j.virusres.2008.02.005",
language = "English",
volume = "135",
pages = "42--47",
journal = "Virus Research",
issn = "0168-1702",
publisher = "Elsevier",
number = "1",

}

Sequence analysis of a reovirus isolated from the winter moth Operophtera brumata (Lepidoptera: Geometridae) and its parasitoid wasp Phobocampe tempestiva (Hymenoptera: Ichneumonidae). / Graham, Robert I.; Rao, Shujing; Sait, Steven M.; Attoui, Houssam; Mertens, Peter P.C.; Hails, Rosemary S.; Possee, Robert D.

In: Virus Research, Vol. 135, No. 1, 07.2008, p. 42-47.

Research output: Contribution to journalJournal Article

TY - JOUR

T1 - Sequence analysis of a reovirus isolated from the winter moth Operophtera brumata (Lepidoptera: Geometridae) and its parasitoid wasp Phobocampe tempestiva (Hymenoptera: Ichneumonidae)

AU - Graham, Robert I.

AU - Rao, Shujing

AU - Sait, Steven M.

AU - Attoui, Houssam

AU - Mertens, Peter P.C.

AU - Hails, Rosemary S.

AU - Possee, Robert D.

PY - 2008/7

Y1 - 2008/7

N2 - A reovirus was isolated from Operophtera brumata (ObRV) and its parasitoid wasp Phobocampe tempestiva. Each of the 10 dsRNA genome segments of ObRV was sequenced and shown to contain a single open reading frame (ORF). Conserved motifs ([+ve] 5′-AAATAAA ... G/TAGGTT-3′) were found at the termini of each segment, with the exception of Seg-6 and Seg-8, where the 5′ termini were 5′-AACAAA...-3′. The putative proteins encoded by each segment were compared with those of other members of the family Reoviridae. Phylogenetic comparisons to published sequences for the RNA-dependent RNA polymerase genes from other reoviruses indicated that ObRV is most closely related to members of the genus Cypovirus. However, unlike the cypoviruses, ObRV has a double-layered capsid structure. When the protein encoded by ObRV Seg-10 was expressed (by inserting the open reading frame into a baculovirus expression vector) no 'occlusion bodies' were observed in the recombinant baculovirus infected insect cell cultures. This suggests that unlike the cypoviruses, Seg-10 of ObRV does not contain a polyhedrin gene. Further phylogenetic comparisons also identified relationships between Seg-2 and Seg-10 of ObRV, and genes of Diadromus pulchellus Idnoreovirus 1 (DpIRV1), suggesting that ObRV represents a new species from the genus Idnoreovirus. © 2008 Elsevier B.V. All rights reserved.

AB - A reovirus was isolated from Operophtera brumata (ObRV) and its parasitoid wasp Phobocampe tempestiva. Each of the 10 dsRNA genome segments of ObRV was sequenced and shown to contain a single open reading frame (ORF). Conserved motifs ([+ve] 5′-AAATAAA ... G/TAGGTT-3′) were found at the termini of each segment, with the exception of Seg-6 and Seg-8, where the 5′ termini were 5′-AACAAA...-3′. The putative proteins encoded by each segment were compared with those of other members of the family Reoviridae. Phylogenetic comparisons to published sequences for the RNA-dependent RNA polymerase genes from other reoviruses indicated that ObRV is most closely related to members of the genus Cypovirus. However, unlike the cypoviruses, ObRV has a double-layered capsid structure. When the protein encoded by ObRV Seg-10 was expressed (by inserting the open reading frame into a baculovirus expression vector) no 'occlusion bodies' were observed in the recombinant baculovirus infected insect cell cultures. This suggests that unlike the cypoviruses, Seg-10 of ObRV does not contain a polyhedrin gene. Further phylogenetic comparisons also identified relationships between Seg-2 and Seg-10 of ObRV, and genes of Diadromus pulchellus Idnoreovirus 1 (DpIRV1), suggesting that ObRV represents a new species from the genus Idnoreovirus. © 2008 Elsevier B.V. All rights reserved.

KW - Operophtera brumata

KW - Protein expression

KW - Reovirus

KW - Winter moth

U2 - 10.1016/j.virusres.2008.02.005

DO - 10.1016/j.virusres.2008.02.005

M3 - Journal Article

VL - 135

SP - 42

EP - 47

JO - Virus Research

JF - Virus Research

SN - 0168-1702

IS - 1

ER -